Resumption of Autophagy by Ubisol-Q₁₀ in Presenilin-1 Mutated Fibroblasts and Transgenic AD Mice: Implications for Inhibition of Senescence and Neuroprotection

Figure 3

Treatment with SP600125 leads to resumption of senescence phenotype and reduced autophagosome formation in Ubisol-Q₁₀-treated cells. (a, b) Immunofluorescence staining of PSAF probing for p21 (green) and LC3 (red) and quantification of fluorescence, respectively. Treatment of PSAF with Ubisol-Q₁₀ leads to increased staining for LC3 puncta an autophagosome indicator. Ubisol-Q₁₀ also lead to reduced staining for p21, an indicator of senescence. Ubisol-Q₁₀-treated PSAF incubated in the presence of SP600125 resulted in increased staining for p21 and reduced staining for LC3 puncta similar to that of untreated PSAF, PSAF treated with SP600125, and PSAF starved of Ubisol-Q₁₀ for 48 hours. Nuclei were counterstained with Hoechst for visualization. Micrographs were taken at 400x magnification. .

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