Resveratrol Attenuates Oxidative Stress-Induced Intestinal Barrier Injury through PI3K/Akt-Mediated Nrf2 Signaling Pathway

Figure 4

Effects of resveratrol and/or H₂O₂ on ROS levels and the activities of T-SOD, CAT, and GSH-Px in IPEC-J2 cells. IPEC-J2 cells were pretreated with RSV at the concentration of 0 μM, 20 μM, and 50 μM for 6 hours and then cocultured with 500 μM H₂O₂ for 4 hours. (a) The fluorescence intensity of ROS was measured by a fluorescence microplate reader. (b–d) Cells were pretreated with or without RSV at the indicated concentrations for 6 h and then incubated in the presence of H₂O₂ at the concentration of 500 μM, and the activities of T-SOD (b), CAT (c), and GSH-Px (d) in cell lysates were detected by a spectrophotometer using commercial kits. Values are the ; . The columns with the same superscript capital letters and with different superscript capital letters mean no significant difference () and significant difference (), respectively.

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(b)

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