Remote Ischemic Preconditioning Protects Cisplatin-Induced Acute Kidney Injury through the PTEN/AKT Signaling Pathway

Figure 3

MiR-144 activates the AKT/GSK3β pathway by directly targeting PTEN (a) The sequence alignment of miR-144 and the 3-UTR of PTEN mRNA. Wild-type (wt) and mutant (mut) sequences were used in (b). (b) Luciferase activities determined the binding between miR-144 and 3-UTR of PTEN. NRK52 cells were cotransfected with the indicated plasmids. Each firefly luciferase activity was normalized by the cotransfected Renilla luciferase activity. ; ; n.s: no significance in statistic. (c) Representative confocal image of immunofluorescence staining with p-GSK3β and cleaved caspase-3 in kidney sections. (d, e) Western blot determined the indicated protein level (d) and their quantification (e). ; ; ; ; n.s: no significance in statistic.

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