Extracellular DNA Containing (dG)n Motifs Penetrates into MCF7 Breast Cancer Cells, Induces the Adaptive Response, and Can Be Expressed

Figure 2

Some characteristics of the plasmids pEGFP and pEGFP-Gn. (a) Plasmid pEGFP-C1 (pEGFP) (http://www.bdbiosciences.com). (2) Distribution of dG₃ and dG₅ motifs within the pEGFP and pEGFP-Gn. Fragment A (red line), used for the analysis of plasmid DNA and RNA EGFP, is indicated. (b) Quantitation of pEGFP and pEGFP-Gn in the cellular DNA after 3, 24, and 72 hours of incubation; pEGFP content (3 h) is taken as one unit. (c) Plasmid content and fragmentation in the extracellular DNA after 3 hours of MCF7 incubation with 100 ng/mL pEGFP or pEGFP-Gn. Electrophoresis of DNA was carried out in a 1% agarose gel stained with ethidium bromide. Gel tracks 4 and 5—plasmids were added to the medium immediately prior to isolation of the cfDNA.

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