Extracellular DNA Containing (dG)n Motifs Penetrates into MCF7 Breast Cancer Cells, Induces the Adaptive Response, and Can Be Expressed

Figure 5

An exposure of the human cultured cells to GC-DNAs leads to an increase in DNA damage. (a) Comet assay in alkaline conditions. Digital photography of the nuclei with varying degree of DNA damage. (b) Cumulative histograms for the tail moment (TM) of the comets. The time of incubation of the cells with GC-DNAs and the type of GC-DNA are shown on the figure. (c) The average of the median (TM) for the comet assay. (d) Cumulative histograms for the tail moment (TM) of the comets (1) and the average of the TM (2). 1a: the cells were incubated for 3 hours in the presence of 50 ng/mL pEGFP-Gn, then pEGFP-Gn was added for 0.5 hours. 1b: the cells were incubated for 3 hours in the presence of 50 ng/mL pEGFP-Gn, then the culture medium was changed, and after 24 hours, the pEGFP-Gn was added for 0.5 hours. .

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