Extracellular DNA Containing (dG)n Motifs Penetrates into MCF7 Breast Cancer Cells, Induces the Adaptive Response, and Can Be Expressed

Figure 6

An exposure to pEGFP-Gn enhances survival in MCF7 cancer cells. (a) Total number of cells in studied cell population. Gray bars: plasmids were added to the cell culture medium and the cells were cultured for 72 hours. Black bars: plasmids were added to the cell culture medium; the cells were incubated for 30 min, then irradiated (10 cGy). After this, the cells were cultured for 72 hours. (b) FCA. (1) Distribution of fluorescence intensities of the cells stained with PI. (2) Proportions of the cells with the amount of DNA corresponding to the sub-G₀/G₁, G₁-, S, and G₂/M phases of the cell cycle. (c) Evaluation of modified nuclei in MCF-7. (1) Example of Hoechst 33342 staining; (2) graph of the proportion of cells with modified nuclei in four studied types of MCF-7 cultures. .

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