Extracellular DNA Containing (dG)n Motifs Penetrates into MCF7 Breast Cancer Cells, Induces the Adaptive Response, and Can Be Expressed

Figure 7

The expression of EGFP in MCF7 treated with pEGFP-Gn or pEGFP for 24 h. (a) FM-based evaluation of EGFP in the cells treated with pEGFP, pEGFP-Gn, or pEGFP-Gn/TurboFect. ; magnification ×20 (left) and 200 (right). (b) FCA. (1, left): nonfixed cell plots: SSC versus FCS; : the analyzed cell subpopulation. (1, right): the distribution of FL1-EGFP fluorescence intensities. and : gated areas. (2) Median signal intensity of FL1-EGFP () and relative proportions of EGFP-positive cells in gate; the conditions for cultivation MCF7 and processing of the plasmids are indicated. (c) FCA. Median signal intensity of FL1-EGFP (R₁) and relative proportions of EGFP-positive cells in gate. MCF7 were irradiated with IR at a dose of 10 cGy. (d) RT-qPCR. The levels of EGFP—encoding RNAs in the cells exposed to pEGFP or pEGFP-Gn. .

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