LncRNA MALAT1 Suppression Protects Endothelium against oxLDL-Induced Inflammation via Inhibiting Expression of MiR-181b Target Gene TOX

Figure 3

Potential binding sites of miR-181b for MALAT1 and TOX. (a, b) The wild-type and mutated miR-181b binding sites in the MALAT1 3-UTR. (c–g) The miR-181b mimics and the luciferase constructs were cotransfected into cultured HUVECs. Cellular lysates from cultured cells were used for RIP with an Ago2 antibody. The Ago2 protein level was detected by Western blot analysis. The mRNA expression of MALAT1 and miR-181b in the immunoprecipitate was measured by RT-PCR. versus the control group (c, d). compared with IgG (e–g). (h, i) The wild-type and mutated miR-181b binding sites in the 3-UTR of TOX. (j, k) Luciferase activity of the wild-type and mutant Luc TOX groups. versus the control group.

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