miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling

Figure 1

miR-154-5p is upregulated during AngII-induced cardiac remodeling. (a) Representative images of mouse hearts and the ratio of heart weight to body weight (HW/BW) in sham- and AngII-treated (2.5 mg/kg per day, 14 days) mice (). In this time point, the heart was in the period of cardiac hypertrophy. (b) Histological analysis of the surface area of mouse cardiomyocytes using WGA staining (). (c) Images of Masson’s trichrome-stained sections of the mouse heart (). (d) Images of TUNEL staining and the average data obtained from heart sections (). (e) Cleaved caspase-3 and caspase-3 expression, as assayed by Western blotting (). (f) Conservation of the miR-154-5p sequence between mice, rats, and humans is shown. (g) The cardiac expression of miR-154-5p in AngII-treated and sham-treated mice. (h) Distribution of miR-154-5p in various tissues from C57BL/6J mice (). miR-154-5p expression is upregulated in a dose-dependent (i) and time-dependent (j) manner in AngII-treated mice (). (k) The surface area of cardiomyocytes (control group and treated 100 nM AngII for 24 h) was identified using α-actinin staining (green), and nuclei were stained with Hoechst33342 (blue) (). (l) Expression of the ANF, BNP, and β-MHC mRNAs in cardiomyocytes (). (m) Images of TUNEL staining in cardiomyocytes and quantitative analysis (). (n) Levels of cleaved caspase-3 and caspase-3 in AngII-treated and control cardiomyocytes (). (o) The expression of miR-154-5p in AngII-treated and control cardiomyocytes (). Data are presented as the . , , and .

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