ALCAT1 Overexpression Affects Supercomplex Formation and Increases ROS in Respiring Mitochondria

Figure 6

ALCAT1 hampers SC formation under stimulated OXPHOS conditions. (a) Fluorescence lifetime images of sEcGFP at subunit CoxVIIIa. This construct is buried in an existing respiratory supercomplex and has a reduced fluorescence lifetime . As a control, subunit CoxIV was labeled with sEcGFP, which does not respond to SC formation by decreased lifetime [28]. Cells were transfected with ALCAT1-mCherry (tr. ALCAT1) and grown in a glucose (5.6 mM) or galactose (10 mM) medium for 48 h. (b) Average fluorescence lifetimes of CoxVIIIa-sEcGFP under different respiratory conditions +/- ALCAT1-mCherry overexpression. (c) Average fluorescence lifetimes of CoxIV-sEcGFP under different respiratory conditions±ALCAT1-mCherry overexpression. replicates. Data was analyzed by One-Way ANOVA with post hoc Scheffe test. Scale bars: 20 μM (a).

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