Research Article

NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis

Figure 1

NOX-derived ROS generation plays an important role in DEX-induced apoptosis of MC3T3-E1 cells. (a) MTT assay analysis of MC3T3-E1 cell viability. (b) The relative ROS content generated was quantified by average fluorescence intensity (d). (c) The quantitative analysis of apoptotic MC3T3-E1 cells (e). (d) After 24 h exposure to DEX, the MC3T3-E1 cells were observed under inverted fluorescence microscopy (×100 magnification). (e) After 24 h exposure to DEX, annexin V-FITC/PI was used for the detection of the rate of apoptosis in MC3T3-E1 cells. ((a) ; (b, c) ; A, compared with the control; B, compared with the DEX group; in the DEX group).
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