NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis

Figure 2

DEX increases NOX1 and NOX4 expression in MC3T3-E1 cells, and the NOX1, NOX2, and NOX4 siRNA efficiencies were detected. (a) Representative immunoblots and densitometric analyses of NOX2. (b) Representative immunoblots and densitometric analyses of NOX4. (c) DEX increased Nox1 mRNA expression. (d) Nox2 mRNA levels induced by DEX. (e) DEX increased Nox4 mRNA expression. (f) RT-PCR detection of Nox1 mRNA in MC3T3-E1 cells transfected with NOX1 siRNA for 48 h. (g) Western blot detection of NOX2 protein expression following 48 h NOX2 siRNA transfection. (h) Western blot detection of NOX4 protein expression following 48 h NOX4 siRNA transfection (; compared with the control; compared with the control; compared with the control; in the DEX group).

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