NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis

<div>NOX1 and NOX4 are involved in DEX-induced ROS production. (a–c) PCR confirmation of NOX1 (a), NOX4 (b), and NOX2 (c) silencing in MC3T3-E1 cells transiently transfected with siRNA (60 nM) for 48 h. (d) DCFH-DA probe detection of intracellular ROS in siRNA-transfected osteoblasts treated with 1000 nM DEX for 24 h. (e) Quantitative analysis of DCF fluorescence intensity in (d) (<span class="nowrap"><svg height="8.55521pt" id="M40" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M285 378C315 398 338 416 353 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis 