Curculigoside Protects against Excess-Iron-Induced Bone Loss by Attenuating Akt-FoxO1-Dependent Oxidative Damage to Mice and Osteoblastic MC3T3-E1 Cells

Figure 3

The antioxidant effect of CUR in iron-overload mice and MC3T3-E1 cells treated with excess iron. (a–d) The values of SOD, MDA, CAT, and GPx in the liver tissue of iron-overload mice assayed by ELISA. (e–h) The levels of SOD, MDA, CAT, and GPx in MC3T3-E1 cells. (i) The inhibitory effect of CUR, NAC, and DFO on the generation of ROS in MC3T3-E1 cells treated with excess iron, upper row: cell vitality; down row: fluorescent intensities of ROS. (j) The protein expression of SOD and CAT in MC3T3-E1 cells. Results are represented as the . ( in animal experiments; in osteoblast experiments), and , compared with the normal control group; ^# and ^##, compared to the model group. N: normal control group, cells were treated with DMSO; M: model group, cells were treated with FAC (500 μM); DFO: cells were treated with DFO (100 μM) and FAC (500 μM); NAC: cells were treated with NAC (1000 μM) and FAC (500 μM); CUR: cells were treated with CUR (10 μM) and FAC (500 μM).

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