Increased Thioredoxin-1 Expression Promotes Cancer Progression and Predicts Poor Prognosis in Patients with Gastric Cancer

Figure 2

Overexpression of Trx-1 promotes gastric cancer cell growth, colony formation, invasion, and migration. (a) The expression level of Trx-1 was analyzed by Western blotting in GES-1, AGS, BGC-823, SGC-7901, and KATO III cell lines. (b) Increased mRNA level of Trx-1 in AGS cells transduced with lenti-Trx-1 using quantitative RT-PCR. (c) Increased protein level of Trx-1 in AGS cells transduced with lenti-Trx-1 revealed by Western blotting. (d) Ectopic expression of Trx-1 promoted AGS cell growth. Cell growth was assessed using the CCK-8 assay. (e) Ectopic expression of Trx-1 promoted AGS cell plate colony formation. (f) Ectopic expression of Trx-1 enhanced AGS cell migration and invasion via the Transwell assay. Representative images from triplicate experiments are shown. Magnification, ×200. The quantitation of migrated and invaded cells is shown in the bottom panel. , .

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