Increased Thioredoxin-1 Expression Promotes Cancer Progression and Predicts Poor Prognosis in Patients with Gastric Cancer

Figure 5

Effects of Trx-1 inhibitor, PX-12, on BGC-823 and AGS cell growth and apoptosis. (a) BGC-823 and AGS cells were treated with PX-12 in increasing concentrations for 24 and 48 hours. 24-hour IC₅₀ = 11.59 μМ, 48-hour IC₅₀ = 8.80 μМ for BGC-823 cells and 24 h IC₅₀ = 6.43 μМ, 48 h IC₅₀ = 4.43 μМ for AGS cells. (b) Gastric cancer cell colony formation is affected by PX-12 treatment. BGC-823 and AGS cells were treated with the indicated concentrations of PX-12 (0, 1, 2.5, and 5 μM) for 48 hours and then changed to medium not containing PX-12 to allow for colony formation for 14 days. (c) Apoptosis analysis by annexin V/PI staining in BGC-823 and AGS cells treated with PX-12 (0, 1, 5, and 10 μM) for 48 hours.

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