Research Article

The Role of Nrf2 Signaling Pathway in Eucommia ulmoides Flavones Regulating Oxidative Stress in the Intestine of Piglets

Figure 2

Cell viability and cytotoxicity. IPEC-J2 cells were treated with DMEM-H medium (control) or with 70 μM paraquat (PQ) or 1.9 μM specific Nrf2 inhibitor (ML385) for 12 h. Cell viability was measured using the Cell Counting Kit-8 from Dojindo Molecular Technologies. Cytotoxicity was determined by lactate dehydrogenase (LDH) release using the LDH Cytotoxicity Assay Kit from Beyotime Institute of Biotechnology. Data expressed as ; independent experiments. Different letters (A, B, and C) indicate significant differences ().