Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells

Figure 4

ISL inhibits the expression of mitoNEET, which regulates ROS, and subsequently induces apoptosis and mitochondrial dysfunction in A375 cells. (a) ISL inhibits the protein expression of mitoNEET in A375 cells detected by western blotting. (b) Relative band intensities of mitoNEET in empty vector- or mitoNEET-overexpressing cells with or without ISL treatment. (c) ISL regulates cellular ROS levels by controlling the mitoNEET expression. (d) ISL induces proliferation inhibition of A375 cells by inhibiting the expression of mitoNEET. (e) ISL decreases mitochondrial membrane potential by inhibiting the expression of mitoNEET in A375 cells. (f) ISL increases the expression of cleaved PARP and cleaved caspase-3 by inhibiting the expression of mitoNEET. (g–h) Relative band intensities of cleaved PARP and cleaved caspase-3 in empty vector- or mitoNEET-overexpressing cells with or without ISL treatment. and versus control; and versus ISL; and and versus vector-Ctrl.

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