Bidens pilosa Extract Administered after Symptom Onset Attenuates Glial Activation, Improves Motor Performance, and Prolongs Survival in a Mouse Model of Amyotrophic Lateral Sclerosis

Figure 7

Effect of MBP on SOD1 protein expression in spinal cord tissue of G93A and WT mice. Mice were orally administered with injection water (vehicle) or MBP, starting at a late symptomatic stage (15 weeks old). Three weeks after the start of the treatment, the lumbar spinal cords were analysed by western blot. Photographs show representative western blot of SOD1 in the lumbar spinal cord of male G93A mice and WT mice. The upper band represents human SOD1 (hSOD1; 21 kDa) and the lower band represents mouse SOD1 (mSOD1; 16 kDa). Equal amounts of cell lysates (10 μg) were analysed, with β-actin as an internal marker. The graph shows the relative density of bands on the blots estimated quantitatively using Scion imaging software. Quantitative data are expressed as the ratio of the band intensity of hSOD1 relative to the band intensity of β-actin. Each value represents the . Statistical significance was determined by unpaired Student’s -test (-7). ND: not detected.