Reactive Oxygen Species Induce Endothelial Differentiation of Liver Cancer Stem-Like Sphere Cells through the Activation of Akt/IKK Signaling Pathway

Figure 7

ROS induced endothelial differentiation of sphere cells through the activation of Akt/IKK signaling pathway. Endothelial differentiated sphere cells cultured in the presence or absence 1 mM NAC for 30 min were further treated with 100 μM H₂O₂ or 1 mM BSO for 24 h. The cells were then subjected to western blotting for detecting the indicated proteins (a, b). Endothelial differentiated sphere cells were seeded on Matrigel in the presence or absence of Akt1/2 kinase inhibitor (10 μM) or BAY 11-7082 (2.5 μM) for 30 min and then further treated with or without 100 μM H₂O₂ (c) or 1 mM BSO (d) for 24 h. The photographs of tube formation were taken (100×). The number of formed tubes was calculated. The data were presented as (). , vs. control group and ^##, ^### vs. H₂O₂ or BSO treatment alone.

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