NOX2 Is Critical to Endocardial to Mesenchymal Transition and Heart Development

<div>Congenital heart defects in <i>Nox2</i><sup>-/-</sup> mice at P0. (a) Representative histological images of normal and abnormal hearts. <i>Nox2</i><sup>-/-</sup> mice exhibited ASD, a complete AVCD (arrows point to interrupted septum primum and VSD), and membranous and muscular (arrows) types of VSD. (b) Representative histological images of tricuspid and mitral valves. (c) Toluidine blue staining of extracellular glycosaminoglycans (light purple) in AV valves. (d) Picrosirius red staining of collagen fibers. (e–g) Quantification of mitral and tricuspid valve length and AV valve thickness. (h) Quantification of the total glycosaminoglycan- (GAG-) positive area in AV valves. 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Scale bar in (a) is 200 <i>μ</i>m and 100 <i>μ</i>m in (b–d).</div>

Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: NOX2 Is Critical to Endocardial to Mesenchymal Transition and Heart Development 