NOX2 Is Critical to Endocardial to Mesenchymal Transition and Heart Development

Figure 3

Expression of NOX2 in hearts of E10.5 embryos. (a) Representative immunostaining of NOX2 in WT hearts. (b) Absence of NOX2 staining in Nox2^-/- hearts. (c, d) Enlarged from boxed areas in (a) showing NOX2 expression (arrows) in the atrial and ventricular myocardium of WT hearts. (e) Arrows indicate NOX2 expression in the myocardium overlying AV endocardial cushion in WT but not Nox2^-/- hearts (f). Lower panels of (e) and (f) are enlarged images of the boxed areas in the upper panels. Scale bar is 100 μm.

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