NOX2 Is Critical to Endocardial to Mesenchymal Transition and Heart Development

Figure 7

Apoptosis and cell proliferation in endocardial cushions. (a) Representative images of histological sections immunostained for cleaved caspase-3 protein at E10.5. Arrows point to positive cells (brown). (b) Quantification of cleaved caspase-3-positive cells at E10.5. (c) Representative images of Ki67 immunostaining in E12.5 hearts. Quantification of Ki67-positive cells (d) and cell density (e) in endocardial cushions at E12.5. Lower panels (scale bars =10 μm) are enlargement of the boxed areas of the upper panels (scale bars =100 μm) in (a, b). hearts per group. vs. WT by unpaired Student’s -test.

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