Research Article
NOX2 Is Critical to Endocardial to Mesenchymal Transition and Heart Development
Table 1
Sequences of primers used for real-time PCR analysis.
| | Gene | Accession No. | Product size (bp) | Primer sequence () |
| | Bmp2 | NM_007553.3 | 151 | F: CAAACACAAACAGCGGAAGC
R: CAGCAAGGGCAAAAGGACAC | | Bmp4 | NM_007554.2 | 250 | F: GTTATGAAGCCCCCAGCAGA
R: CCCAATCTCCACTCCCTTGA | | Gata4 | NM_008092.3 | 134 | F: GCCTGCGATGTCTGAGTGAC
R: CACTATGGGCACAGCAGCTC | | Gata5 | NM_008093.2 | 167 | F: ACCCCACAACCTACCCAGCA
R: GCCCTCACCAGGGAACTCCT | | Mef2c | NM_001170537.1 | 405 | F: CACCGAGTACAACGAGCCGCA
R: CTGGTGCCTGCACCGGATGTC | | Nkx2.5 | NM_008700.2 | 162 | F: GACAGCGGCAGGACCAGACT
R: CGTTGTAGCCATAGGCATTG | | Snail1 | NM_011427.2 | 114 | F: CACACGCTGCCTTGTGTCT
R: GGTCAGCAAAAGCACGGTT | | Tbx5 | NM_011537.3 | 103 | F: AGGAGCACAGTGAGGCACAA
R: GGGCCAGAGACACCATTCTC | | Tgf-β2 | NM_009367.3 | 230 | F: CTGTGCAGGAGTGGCTTCAC
R: GCAGGAGATGTGGGGTCTTC | | Notch1 | NM_008714.3 | 142 | F: CAGCTTGCACAACCAGACAGA
R: TAACGGAGTACGGCCCATGT | | 28S | NR_003279.1 | 178 | F: GGGCCACTTTTGGTAAGCAG
R: TTGATTCGGCAGGTGAGTTG |
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F and R indicate forward and reverse primers, respectively.
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