Lipin1 Is Involved in the Pathogenesis of Diabetic Encephalopathy through the PKD/Limk/Cofilin Signaling Pathway

Figure 1

Changes in PC12 cell viability in response to high glucose toxicity are related to Lipin1. (a) Cell viability after 24 or 48 h of treatment with different concentrations of glucose and mannitol, and the cells were incubated in the same concentrations (100 mM) of glucose and mannitol for 48 h. The cell viability of each group was standardized to that of the control group. (b) Incubated with glucose, lipin1 mRNA and protein expressions as determined using qPCR and Western blot. (c) Incubated with mannitol, lipin1 mRNA and protein expressions as determined using qPCR and Western blot. , , versus 25 mM/48 h. (d) Cells infected with Lv-Lipin1, Lv-Lipin1-Con, Lv-Lipin1ShRNA, and Lv-Lipin1ShRNA-Con for three days. Nucleus were stained blue with 4,6-diamidine-2-phenylidole dihydrochloride (DAPI). Infection efficiencies were determined using fluorescence microscopy while postinfection Lipin1 mRNA and protein expressions were determined using qPCR and Western blot analysis. , , versus LV-Lipin1ShRNA–Con group; ^#, ^### versus LV-Lipin1-Con group. (e) Determinations of viability in cells transfected with different viruses under different treatment conditions. , versus NG+LV-Con group; ^##, versus HG+LV-Con goup. . Data are expressed as . Results are representative of three independent experiments.

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