Klotho Alleviates Lung Injury Caused by Paraquat via Suppressing ROS/P38 MAPK-Regulated Inflammatory Responses and Apoptosis

Figure 5

NAC treatment reduces intracellular ROS level and P38 MAPK activation in PQ-exposed A549 cells. (a) ROS production in A549 cells was measured by DCFH-DA staining under a fluorescence microscope. (b) Quantification of the DCF fluorescence intensity was conducted by Image-Pro Plus software. The expression of phosphorylated P38 MAPK was detected by western blotting (c) and quantified by the relative intensity of protein bands (d).

(a)

(b)

(c)

(d)