Circulating Levels of CILP2 Are Elevated in Coronary Heart Disease and Associated with Atherosclerosis

<div>Transcriptional regulation of CD36 promoter by CILP2 <i>via</i> PPAR<i>γ</i> pathway. (a) CILP2-mediated transcription of CD36 promoter was blocked by GW9662 in THP-1 cells. (b) Deletion analysis of CD36 promoter. THP-1 cells were transfected with CILP2 overexpression vector, together with a series of truncated CD36 promoter-driven luciferase reporters. 48 h after the transfection, the luciferase activity was measured and expressed in relative luciferase units. (c) Site-directed mutagenesis analysis. THP-1 cells were cotransfected with the CILP2 expression and luciferase reporter plasmids containing WT and PPRE-G or PPRE-J binding site mutant CD36 promoters, and luciferase activity was measured. 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Oxidative Medicine and Cellular Longevity

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Figure 5

Figure 5: Circulating Levels of CILP2 Are Elevated in Coronary Heart Disease and Associated with Atherosclerosis 