HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration

Figure 1

HSF1 reduced lung tissue injury and improved the outcome of LPS-induced ALI mice. (a) Effect of HSF1 on macroscopic changes at 12 h, 24 h, and 36 h after LPS treatment of ALI mice. (b) Representative morphological changes of the lung tissue from LPS-induced ALI mice. Arrows show pulmonary edema, inflammatory cell infiltration, and alveolar hemorrhage (HE staining, ×20 magnification). (c) The lung W/D ratio after 24 h of LPS treatment of ALI mice. (d) The total protein content in BALF from LPS-induced ALI mice; ^#, ^##, versus HSF1^+/++NS group; , versus HSF1^+/++LPS group; mice per group. (e) The survival rate of LPS-induced ALI mice expressed as a percentage. The survival rate was significantly lower in the HSF1^-/- group than in wild-type mice (20.0% versus 53.3%) after 72 h of LPS treatment; mice per group; , versus HSF1^+/+ group. values were determined using two-tailed Student’s -test for comparing two groups and one-way ANOVA for comparing multiple groups.

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