HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration

Figure 2

HSF1 reduced macrophage infiltration into the lung tissue from LPS-induced ALI mice. (a)–(d) BALF weas harvested and analyzed for macrophage accumulation using flow cytometry to detect CD45⁺ and F4/80⁺ cells at 12 h (a), 24 h (b), and 36 h (c) after LPS treatment. (d) Statistical analysis of (a), (b), (c), respectively. (e) Effect of HSF1 on macrophage infiltration into the lung tissue at 12 h, 24 h, and 36 h after LPS treatment in ALI mice (F4/80, green; white arrowheads indicate individual macrophages; immunofluorescence staining, ×40 magnification); ^#, ^##, versus HSF1^+/++NS group; , , versus HSF1^+/++LPS group; mice per group. values were determined using two-tailed Student’s -test for comparing two groups and one-way ANOVA for comparing multiple groups.

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