HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration

Figure 4

HSF1 reduced the CCR2 expression in macrophages from LPS-induced ALI mice. (a)–(d) BALF samples were analyzed using flow cytometry to measure the CCR2 expression in macrophages (CD45⁺, F4/80⁺, CCR2⁺) at 12 h (a), 24 h (b), and 36 h (c) after LPS treatment. (d) Statistical analysis of (a), (b), and (c). (e)–(g) Immunofluorescence staining of the CCR2 expression (red) in macrophages (green) from the lung tissue at 12 h (e), 24 h (f), and 36 h (g) after LPS treatment (F4/80, green; CCR2, red; DAPI, blue; yellow dots indicated by white arrowheads represent the CCR2 expression in macrophages; immunofluorescence staining, ×40 magnification). (h) CCR2 mRNA levels in the lung tissue from LPS-induced ALI mice using qRT-PCR. ^#, ^##, versus HSF1^+/++NS group; , , versus HSF1^+/++LPS group; mice per group. values were determined using two-tailed Student’s -test for comparing two groups and one-way ANOVA for comparing multiple groups.

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