The Reduced Oligomerization of MAVS Mediated by ROS Enhances the Cellular Radioresistance

Figure 3

Effects of MAVS ectopic expression on cell apoptosis and the changes in regulatory proteins induced in negative vector and MAVS-silenced cells after irradiation. (a) The expression of apoptosis-related proteins was assessed and quantified in negative vector or MAVS-silenced A549 cells after 2 Gy X-ray irradiation by western blotting. (b) Value of Bcl2/Bax protein expression quantified using ImageJ software was presented. (c) Value of full length/cleaved caspase-3 protein expression quantified using ImageJ software was presented. (d) Apoptosis was quantified by the combined staining of Annexin V and PI, and fluorescence was analyzed using flow cytometry in negative vector and MAVS-silenced A549 cells after irradiation. Each data point represents the mean of three separate experiments. Values are presented as . , , and vs. the control group; , , and vs. the irradiation group.

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