The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes

Figure 3

EGT suppressed UVA-induced ROS production in HSF cells. Cells were pretreated with EGT (0.125-0.5 μM) for 24 h and then irradiated with 3 J/cm² UVA. DCF showed intracellular ROS levels and were measured by fluorescence microscopy (200x magnification). 30 min before each experiment was complete, the nonfluorescent, cell membrane-permeable probe DCFH₂-DA was added to the culture medium with a final concentration of 10 μM. The cells were penetrated by DCFH₂-DA and then reacted with cellular ROS for metabolization into fluorescent DCF. We quantified the percentage of the fluorescence intensity of the DCF-stained cells with Olympus Soft Imaging Solutions for each condition. Data were presented as of three or more assays. compared with untreated control cells and ^## and ^### compared with UVA-irradiated cells.