Dexmedetomidine Attenuates LPS-Induced Monocyte-Endothelial Adherence via Inhibiting Cx43/PKC-α/NOX2/ROS Signaling Pathway in Monocytes

Figure 3

In U937 monocytes, inhibiting PKC-α with Gö6976 attenuates NOX2/ROS signaling pathway, and its downstream VAL-4 and LFA-1 expressions, ultimately resulting in U937-HUVEC adhesion decrease. (a) Gö6976 reduces PKC-α expression in U937 monocytes (, vs. control; ^# vs. the LPS group). (b) Effects of Gö6976 on NOX2 expression in U937 monocytes (, vs. control; ^# vs. the LPS group). (c) Effects of Gö6976 on the level of ROS in U937 monocytes (, vs. control; ^# vs. the LPS group). (d) Effects of Gö6976 on VLA-4 and LFA-1 expressions in U937 monocytes (, vs. control; ^# vs. the LPS group). (e) Effects of Gö6976 on U937-HUVEC adhesion (, vs. control; ^# vs. the LPS group). (f) Effects of dexmedetomidine on PKC-α expression in U937 monocytes (, vs. control; ^# vs. the LPS group).

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