Cardioprotective Effects of Taurisolo® in Cardiomyoblast H9c2 Cells under High-Glucose and Trimethylamine N-Oxide Treatment via De Novo Sphingolipid Synthesis

Figure 2

Taurisolo® increased the nitric oxide values in the medium of THG-treated H9c2 cells. The values of NO were evaluated by the Griess assay. H9c2 cells were grown in standard (5.5 mM NG-H9c2) and high glucose (44 mM Glu, HG-H9c2) condition. The NG and HG-H9c2 cells were treated, for 72 h with Tau (0.5 μg/μL), TMAO (50 μM), and TMAO-Tau combination. Absorbance was assayed at 550 nm and compared with a standard curve obtained using sodium nitrite. TBARS were quantified by spectrophotometry at 532 nm. Results were expressed as TBARS M/g of serum proteins. Each data point is the average of triplicate measurements, with each experiment performed in triplicate. Results are expressed as .