Cardioprotective Effects of Taurisolo® in Cardiomyoblast H9c2 Cells under High-Glucose and Trimethylamine N-Oxide Treatment via De Novo Sphingolipid Synthesis

Figure 4

Taurisolo® modulated ERK activation. H9c2 cells were grown in standard (5.5 mM Glu NG-H9c2) and high glucose (44 mM Glu, HG9c2) condition. The NG and HG-H9c2 cells were treated, for 72 h with Tau (0.5 μg/μL), TMAO (50 μM), and TMAO-Tau combination. (a) Bar graphs show densitometrically quantified ratio of p-ERK/ERK in H9c2 cells. (b) Representative immunoblots of NG-H9c2 cells treated with Tau, TMAO, and Tau-TMAO combination. (c) Representative immunoblots of HG-H9c2 cells treated with Tau, TMAO, and Tau-TMAO combination. Each data point is the average of triplicate measurements, with each experiment performed in triplicate. Results are expressed as .

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