Antitumor Therapy under Hypoxic Microenvironment by the Combination of 2-Methoxyestradiol and Sodium Dichloroacetate on Human Non-Small-Cell Lung Cancer

Figure 6

Representative micrographs of HIF-2α expression in A549 cells under hypoxia treated with DCA, 2-ME, or the combination. (a) The transcription factor is shown in green and nuclei in blue. cells per well in normoxia and cells per well in hypoxia were cultured in four-well chamber slides for 72 h with their respective treatments: DCA 40 mM, 2-ME 10 μM, DCA 40 mM/2-ME 10 μM combination, and control without treatment (2% FBS). Quantification of fluorescence was performed in nuclei: (b) hypoxia and (c) normoxia. cells. , , and .

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