Research Article
Zerumbone Promotes Cytotoxicity in Human Malignant Glioblastoma Cells through Reactive Oxygen Species (ROS) Generation
Table 1
Primer sequences for PCR analysis of apoptotic-related genes.
| Gene symbol | Gene name | Primers (5 ⟶ 3) | Accession number | Product length |
| Bax | Bcl-2-associated X protein | Forward: GGAGCTGCAGAGGATGATTG | NM_138761.4 | 100 | Reverse: CCAGTTGAAGTTGCCGTCAC |
| Bcl-2 | B-cell lymphoma 2 | Forward: CTGAGGAGCTTTGTTTCAACCA | NM_000633.2 | 100 | Reverse: TCAAGAAACAAGGTCAAAGGGA |
| P53 | Tumor suppressor protein | Forward: ACCCTTGCTTGCAATAGGTG | NM_000546.5 | 100 | Reverse: AACAAAACACCAGTGCAGGC |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Forward: ACAACTTTGGTATCGTGGAAGG | NM_002046.7 | 101 | Reverse: GCCATCACGCCACAGTTTC |
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