UFM1-Specific Ligase 1 Ligating Enzyme 1 Mediates Milk Protein and Fat Synthesis-Related Gene Expression via the JNK Signaling Pathway in Mouse Mammary Epithelial Cells

Figure 2

The efficiency of UFL1 in the mouse mammary. In this research, UFL1 KO mice and siUFL1 were used for the established UFL1 deficiency model. (a–c) The mammary was collected from UFL1 pregnant KO mice. (a, b) The expression of UFL1 was determined by western blot in UFL1 KO mice. (c) Relative expressions of UFL1 were tested by qPCR in UFL1 KO mice. (d–f) HC11 cells were transfected with UFL1 siRNA or control. (d, e) Using western blot for the expression of UFL1 or GAPDH in cell lysates. (f) Relative expressions of UFL1 were confirmed by qPCR in HC11 cells, followed UFL1 siRNA transduced. All experiments were tested three times. Graphs were expressed as the of 3 independent experiments. indicates significant difference ().

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