The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission

<div>Role of MLC phosphorylation in Cal-mediated effects on vascular reactivity after THS: (a) representative immunoblots showing the expression and phosphorylation of MLC; (b) ratio of phosphorylated MLC/total MLC based on optical density of immunoblot bands; (c) ratio of total MLC/<i>β</i>-actin based on optical density of immunoblot bands. Immunoblot analyses were repeated three times. (d) Contractile response of superior mesenteric arteries to norepinephrine (NE) (<span class="nowrap"><svg height="12.6917pt" id="M36" style="vertical-align:-3.40337pt" version="1.1" viewbox="-0.0498162 -9.28833 64.1776 12.6917" width="64.1776pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M249 635C141 635 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Sham: sham-operated; LR: lactated Ringer’s solution; Cal: Calhex-231.</div>

Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission 

Figure 7 | The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission 