Research Article

The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission

Figure 8

Effect of Calhex-231 on mitochondrial fission/fusion and mitochondrial morphology following THS: (a) representative immunoblots showing expression of Drp1, Fis1, Mfn1, and Mfn2; (b–e) protein levels normalized to β-actin based on optical density of immunoblot bands. Immunoblot analyses were repeated three times. (f) Representative confocal images showing morphology of rat VSMC mitochondria stained with MitoTracker deep red. Red, MitoTracker fluorescence; blue, DAPI fluorescence. The boxed areas in the top images are shown at higher magnification in the bottom images. Experiments were repeated three times. compared with the sham-operated group; #, ## compared with the LR group. Sham: sham-operated; LR: lactated Ringer’s solution; Cal: Calhex-231; Norm: normal; Hyp: hypoxia.
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