Research Article
The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission
Figure 9
Role of miR-208a in Cal-regulation of mitochondrial fission proteins in hypoxic-VSMCs: (a) effect of miR-208a on the protein expression of Drp1 and Fis1 in hypoxic-VSMCs; (d) role of miR-208a in the regulation of the Drp1 and Fis1 expression by Cal. Representative immunoblots showing expression of Drp1 and Fis1. (b, c, e, and f) Protein levels normalized to β-actin based on optical density of immunoblot bands. Immunoblot analyses were repeated three times. compared with the normal group; # compared with the hypoxia group; ^ compared with the hypoxia+Cal group. Norm: normal; Hyp: hypoxia; miR-208a: miR-208a mimic; miR-208ai: miR-208a inhibitor; Cal: Calhex-231.
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