The Attenuating Effect of the Intraovarian Bone Morphogenetic Protein 4 on Age-Related Endoplasmic Reticulum Stress in Chicken Follicular Cells

Figure 3

GCs dominated follicular ER stress in chicken PHFs. Morphology of follicles ((a1) A and B, (b1) A and B) after 72 h culture. Changes in the TUNEL index in SWFs ((a1) C and D, (b1) C and D) after coculture with GCs. Green: apoptotic cells. BrdU was used to label the cells ((a1) E and F, (b1) E and F) that were proliferating (red). After coculture of the D580-SYF-GCs with D280-SWFs, ER stress marker GRP78 ((a1) G and H) was increased in the GCs. After coculture of the D580-SWFs with D280-SYF-GCs, the ER marker GRP78 ((b1) G and H) was reduced in both GCs and TCs. Scale bar: 20 μm. Western blot and grey analysis of BCL2, p-PERK, ATF4, caspase12, ASK1, and GRP78 (a2) expression in D280-SWFs with D580-SYF-GCs in coculture. Western blot and grey analysis of BCL2, p-PERK, ATF4, caspase12, ASK1, and GRP78 (b2) expression in D580-SWFs with D280-SYF-GCs in coculture. By qRT-PCR analysis, CALR, PERK, GRP78, and CHOP mRNAs (a3) increased in D280-SWFs (coculture with D580-SYF-GCs). The mRNAs of CALR, PERK, GRP78, and CHOP (b3) decreased in D580-SWFs (coculture with D280-SYF-GCs). Values were the of three experiments. Different lowercase letters indicated significant difference ().

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