Research Article

Anti-Diabetic Atherosclerosis by Inhibiting High Glucose-Induced Vascular Smooth Muscle Cell Proliferation via Pin1/BRD4 Pathway

Figure 3

Effect of juglone and JQ1 treatment on Pin1, BRD4, cyclin D1, and MMP-9 protein levels in the thoracic aorta of diabetic Apoe-/- mice. (a) Immunoblot analysis was performed on homogenates from thoracic aorta tissue of C57BL/6 mice, Apoe-/- mice, and diabetic Apoe-/- mice treated with vehicle, juglone, or JQ1. Pin1, BRD4, cyclin D1, and MMP-9 protein levels were determined by western blotting. (b-e) Relative ratios of Pin1, BRD4, cyclin D1, and MMP-9 over β-actin were determined by densitometric analysis. Values are mean ± SEM (n =10 mice per group). vs. C57BL/6; #P <0.05 vs. Apoe-/-;&P <0.05 vs. STZ-Apoe-/-; §P <0.05 vs. STZ-Apoe-/- + juglone.
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