Anti-Diabetic Atherosclerosis by Inhibiting High Glucose-Induced Vascular Smooth Muscle Cell Proliferation via Pin1/BRD4 Pathway

Figure 4

Effect of juglone and JQ1 treatment on Pin1, BRD4, cyclin D1, and MMP-9 protein expression in high glucose-induced VSMCs. (a) Effects of juglone and JQ1 treatment on Pin1, BRD4, cyclin D1, and MMP-9 protein expression in high glucose-cultured VSMCs. (b-e) Relative ratios of Pin1, BRD4, cyclin D1, and MMP-9 over β-actin were determined by densitometric analysis. Values are mean ± SEM (n =6, 6 experiments per group). HG, high glucose (25 mM). vs. blank control group (normal glucose, 5.5 mM); ^#P <0.05 vs. hypertonic group (normal glucose 5.5 mM + mannitol 19.5 mM); ^&P <0.05 vs. HG (25 mM); ^§P <0.05 vs. HG + juglone 10^-5 M.

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