Research Article
Anti-Diabetic Atherosclerosis by Inhibiting High Glucose-Induced Vascular Smooth Muscle Cell Proliferation via Pin1/BRD4 Pathway
Figure 5
Effect of juglone and JQ1 treatment on proliferation and migration of VSMCs induced by high glucose. (a) Effects of juglone and JQ1 treatment on proliferation of VSMCs induced by high glucose. VSMCs were inoculated in 96-well plates. When there was 60%–70% confluence, M199 medium containing 0.3% FBS was replaced and cultured for 24 h. Pretreatment with juglone (10−5 M) or JQ1 (10−5 M) was performed for 45 min, and then high glucose (25 mM) was added with incubation for 24 h. After this time, 10 μL MTT was added and incubated at 37°C for 4–5 h. Optical density was detected at 490 nm. (b, c) Effects of juglone and JQ1 treatment on migration of VSMCs induced by high glucose. Vehicle or high glucose (25 mM) was added to cells with or without the Pin1 inhibitor juglone (10−5 M) or the BRD4 inhibitor JQ1 (10−5 M) to the lower chamber. After 24 h of incubation at 37°C in a 5% CO2 incubator, the cell migration membrane was fixed with methanol and stained with toluidine blue. Cell migration was observed under a microscope (scale bars =50 μm). Values are mean ± SEM (n =6, 6 experiments per group). vs. Control; #P <0.05 vs. HG (25 mM).
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