Maternal Separation Induces Long-Term Alterations in the Cardiac Oxytocin Receptor and Cystathionine γ-Lyase Expression in Mice

Figure 3

Western blot and immunohistochemical analyses of cardiac Oxtr and CSE expression in the LTSS paradigm. Representative Western blot of Oxtr protein expression (a) and quantitative results (b) revealed significantly reduced Oxtr expression in cardiac tissue of LTSS-exposed animals. Oxtr (d) and CSE (f) expression was significantly reduced in LTSS-exposed animals compared to their respective controls in immunohistological staining. Corresponding exemplary pictures of the LV myocardium are shown in (c, e). Data given as box plots (median, interquartile range, minimum, and maximum). ⁺Extreme value; Oxtr: oxytocin receptor; CSE: cystathionine γ-lyase; LTSS: long-term separation stress; CON: control; LV: left ventricular.

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