Vitamin B17 Ameliorates Methotrexate-Induced Reproductive Toxicity, Oxidative Stress, and Testicular Injury in Male Rats

Figure 4

(a–f) Photomicrographs of rat testes sections in the different experimental groups stained with haematoxylin and eosin. (a, b) Testis sections in control and VitB17 groups revealed normal structure of seminiferous tubules with regular cycle of spermatogenesis and the lumen full of with sperms (Sp). (c, d) Testis sections in treated rat with methotrexate revealed disturbance and abnormal arrangement of spermatogenesis cycles (black arrows) and significance decrease in sperms and Leydig cells (white arrows). (e) Testicular section in VitB17+MTX revealed moderate increase in both sperms and Leydig cells (white arrows). (f) Testis section in MTX+VitB17 revealed mild increase in sperm numbers and few Leydig cell numbers (white arrows).

(a)

(b)

(c)

(d)

(e)

(f)