PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease

Figure 3

Effect of Comp23 on the H₂O₂-induced cell death of FHs71Int cells. FHs74Int duodenal epithelial cells were treated with different concentrations of Comp23 in the absence or presence of H₂O₂ (1000 μM); then, oxidative stress-induced cytotoxicity was determined by MTT (a), LDH (b), and Annexin V apoptosis ((c, d); 0.001 μM Comp23) assays. Relative mRNA expressions (e) at 0.001 μM Comp23 concentration were determined by comparison with 18S ribosomal RNA as an internal control. Results are presented as ((a, b, e): ; (c): ). vs. control+Ø compound ((a, b, c): multiple -test; (e): Mann-Whitney test); vs. H₂O₂+Ø compound ((a, b, c): two-way ANOVA; (e): Mann-Whitney test).

(a)

(b)

(c)

(d)

(e)