MUTYH Deficiency Is Associated with Attenuated Pulmonary Fibrosis in a Bleomycin-Induced Model

Figure 1

The bleomycin- (BLM-) induced pulmonary fibrosis mouse model was evaluated using Micro-CT and lung weight/body weight ratios. (a) Schematic representation of the experimental design is shown. (b) Micro-CT and 3D digital reconstruction were employed to directly observe the fibrotic lesions in the lungs of mice on day 28 after intratracheal administration with a single dose of BLM or normal saline (NS) on day 0. (c) Lung weight/body weight ratios are shown compared to NS groups. BLM treatment markedly increased the ratios at all three time points. On day 28, BLM-Mutyh^-/- mice exhibited a significantly lower ratio of lung weight/body weight, compared to BLM-Mutyh^+/+ mice (, ). Statistical significance was analyzed using two-way ANOVA and followed by the LSD post hoc test. represents and # represents compared with the NS-Mutyh^+/+ group. & represents compared to the BLM-Mutyh^+/+ group.

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