MUTYH Deficiency Is Associated with Attenuated Pulmonary Fibrosis in a Bleomycin-Induced Model

Figure 2

MUTYH-deficient mice exhibited attenuated pulmonary fibrosis in the BLM-induced model. (a) Representative histological sections of pulmonary tissues were obtained from experimental animals and subjected to HE staining. (b) The inflammatory score based on HE staining in the BLM-Mutyh^-/- group was significantly lower than that in BLM-Mutyh^+/+ among D7 mice (). (c, d) Representative images of Masson’s trichrome staining in pulmonary tissue sections revealed significantly decreased collagen deposition (blue) in BLM-Mutyh^-/- compared to BLM-Mutyh^+/+ mice among the D28 group (). Scale bars: 100 μm. (e) Collagen deposition was assessed by hydroxyproline contents. BLM-Mutyh^-/- exhibited significantly lower contents compared to BLM-Mutyh^+/+ mice among the D28 group (). mice per group. Statistical significance was analyzed using two-way ANOVA and followed by the LSD post hoc test. represents and # represents compared with NS-Mutyh^+/+ mice. & represents compared to the BLM-Mutyh^+/+ group.

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